Self-Organizing 3D Dermal Papilla Cell Spheroids Yield Therapeutic Extracellular Vesicles That Target Hypertrophic Scar Regression via the miR-26a-5p/CCNE2 Axis

    July 2025 in “ Burns & Trauma ”
    Yunwei Wang, Luyang Zhao, Hao Ma, Ao Shi, Peng Cao, Feiyu Cai, Ruomei Zhao, Gang Wang, Zhihan Hu, Jiatong Wang, Yuchen Kang, Xiaoyu Di, Qingyi Zhang, Hao Zhang, Shuguang Hou, Babo Zhang, Han Wang, Yi Liu, Hao Guan
    TLDR 3D cell spheroids can help reduce scars by delivering therapeutic vesicles.
    The study explores the use of self-organizing 3D dermal papilla cell spheroids to produce therapeutic extracellular vesicles (EVs) that target hypertrophic scar regression. The researchers developed a self-feeder layer 3D (SFL-3D) platform to enhance the functionality and scalability of dermal papilla cells (DPCs), overcoming limitations like rapid replicative senescence. The EVs derived from these cells, enriched with miR-26a-5p, were shown to reduce fibrosis by silencing CCNE2 and suppressing myofibroblast differentiation, effectively managing hypertrophic scars in a rabbit ear model. This scalable acellular therapy offers a promising precision-targeted strategy for hypertrophic scar management, leveraging the miR-26a-5p/CCNE2/PI3K/AKT axis.
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