Pooled, Frozen, Gamma-Irradiated Amniotic Fluid Enhances Histomorphological Remodeling in Hypertrophic Scars

The study demonstrates that pooled, frozen, gamma-irradiated human amniotic fluid (PFI-hAF) significantly enhances the healing of hypertrophic scars in a rat model. PFI-hAF accelerates the remodeling process, leading to reduced fibrosis, improved tissue organization, and increased hair follicle density compared to control groups. It promotes reepithelialization, enhances M2 macrophage activity, and improves collagen organization, resulting in more organized collagen structures and reduced scar width. These findings suggest that PFI-hAF is a promising, minimally manipulated treatment for wound care, offering practical advantages such as room-temperature stability and reduced risk of contamination, with potential applications in regenerative medicine.