Hair Follicle Seedling Cryomicroneedles from Hierarchical Microfluidic Organoid on a Chip

The study introduces innovative hair follicle seedling cryomicroneedles using a hierarchical microfluidic organoid-on-a-chip to enhance hair regeneration. This technology addresses challenges in producing uniform hair follicle organoids (HFOs) with high growth potential and efficient transplantation. The integrated chip facilitates uniform cell distribution into needle-shaped microwells, forming HFOs that are encapsulated with cryopreservation agents. The resulting cryomicroneedles maintain HFO morphology, viability, and hair growth potential, while also ensuring good skin penetration and biosafety. Animal experiments showed that these organoids develop into fully functional hair follicles, suggesting significant potential for human hair follicle reconstruction.