The Impact of Centrifugation Devices and Collection Tubes on Fibrin Characteristics and Growth Factor Release Under High- and Low-Speed Protocols

    October 2025 in “ Dentistry Journal ”
    Oranit Bunyatratchata, Wutigri Nimlamool, Supatra Sang-in
    TLDR Low-speed protocols with certified centrifuges and FDA-approved glass tubes are best for effective PRF outcomes.
    This study examined the impact of centrifugation devices and collection tubes on platelet-rich fibrin (PRF) characteristics and growth factor release using blood samples from six healthy female volunteers. It compared three centrifuges (IntraSpin, Duo, and LMC-3000) and three tube types (Pyrex, A-P, and silica-coated plastic) under high- and low-speed protocols. High-speed protocols produced larger clots, while low-speed protocols resulted in more biologically active matrices. The IntraSpin and Duo centrifuges were more effective in generating larger clots and higher growth factor release compared to the LMC-3000. Tube type did not significantly affect growth factor levels, although silica-coated tubes produced the largest clots, and Pyrex tubes showed superior growth factor release. The study concluded that PRF quality is influenced by centrifuge design, g-force, and tube material, recommending low-speed protocols with certified centrifuges and FDA-approved glass tubes for consistent and effective regenerative outcomes. Limitations include the small sample size, suggesting future studies should include more diverse cohorts.
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