In Vitro Investigation of the Follicular Penetration of Porcine Ear Skin Using a Nanoparticle-Emulsion Containing the Antiseptic Polihexanide

The study investigated the follicular penetration of a curcumin-labeled particle-associated antiseptic, specifically polihexanide, into porcine skin using laser scanning microscopy. It was found that hair follicles serve as bacterial reservoirs, making them important targets for antiseptics. The research compared the penetration depth of particles with and without the antiseptic in vitro using porcine skin biopsies. Results showed that both types of particles penetrated efficiently into hair follicles, but those with the antiseptic had a significantly greater penetration depth. The study also demonstrated that laser scanning microscopy was an effective method for visualizing skin structures and localizing fluorescent markers within the skin and hair follicles.