Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations

January 2018 in “ Journal of Investigative Dermatology ”

Christina Philippeos, Stéphanie B. Telerman, Bénédicte Oulès, Angela Oliveira Pisco, Tanya J. Shaw, Raúl Elgueta, Giovanna Lombardi, Ryan R. Driskell, Mark Soldin, Magnus Lynch, Fiona M. Watt

fibroblast Wnt signaling IFN-γ epidermal reconstitution wound healing fibrotic diseases interferon gamma

TLDR Researchers found four distinct fibroblast types in human skin, which could help in treating wounds and fibrotic diseases.

The study identified at least four distinct subpopulations of human dermal fibroblasts using spatial and single-cell transcriptional profiling. These subpopulations were characterized by specific markers and maintained functional differences, such as variations in Wnt signaling and immune response. The research suggested potential therapeutic applications in wound healing and diseases with excessive fibrosis by targeting specific fibroblast subpopulations. The study also highlighted the evolutionary conservation of gene expression patterns between mouse and human dermis, particularly in the Wnt signaling pathway, and emphasized the heterogeneity of fibroblasts and their implications for skin biology and regenerative medicine.

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