Tri-Layer Gradient Fiber-Aligned Electrospun Composite: Accelerating Skin Wound Healing

The study presents a tri-layer gradient fiber-aligned electrospun composite dressing designed to accelerate skin wound healing by mimicking the skin's natural hydrophilic-hydrophobic gradient. The dressing consists of three layers: a hydrophilic inner layer (A100) for moisture retention and cell growth, a middle layer (A300) for nutrient and gas exchange, and an outer layer (A500) that acts as a mechanical barrier against bacteria. The composite is made from gelatin and chitosan, offering biocompatibility and antimicrobial properties. In vitro and in vivo experiments confirmed the dressing's effectiveness in promoting wound healing, collagen deposition, angiogenesis, hair follicle regeneration, and reducing fibrosis. The dressing is cost-effective, easy to manufacture, and provides a promising method for full-thickness wound healing in skin tissue engineering.