Upcycled Postbiotic Cell-Free Supernatants from Limosilactobacillus Fermentum MG901 and MG4237 Alleviated Oxidative Stress-Induced Dysfunction in Human Follicle Dermal Papilla Cells

The study examines the effects of upcycled postbiotic cell-free supernatants (CFS) from Limosilactobacillus fermentum strains MG901 and MG4237 on human follicle dermal papilla cells under oxidative stress, a condition linked to hair loss. The CFSs, derived from fermentation byproducts, showed no cytotoxicity and improved cell viability, wound-healing capacity, and mitochondrial function while reducing reactive oxygen species levels. They also enhanced alkaline phosphatase activity and activated Wnt/β-catenin signaling, crucial for hair growth. These findings suggest that these CFSs could be used as sustainable cosmetic ingredients to alleviate hair loss. The experiments were conducted in triplicate to ensure reliability.