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GlossaryGel Electrophoresis

Gel electrophoresis is a laboratory technique used to separate DNA, RNA, or proteins based on their size and charge. In the context of hair loss research, it is relevant for analyzing genetic markers associated with hair loss conditions, as well as for studying the expression of specific proteins involved in hair follicle biology. Key distinguishing characteristics include the use of a gel matrix, typically agarose or polyacrylamide, which allows for the migration of molecules when an electric current is applied, enabling researchers to visualize and compare genetic or protein samples.

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Electrophoretic Variability in Human Head Hair: Polyacrylamide Gel Electrophoresis of Hair Proteins in the Presence of Sodium Dodecyl Sulfate and Urea

research Electrophoretic variability in human head hair: Polyacrylamide gel electrophoresis of hair proteins in the presence of sodium dodecyl sulfate and urea

15 citations , January 1987 in “Electrophoresis”

Human head hair proteins can be typed into eight distinct patterns, useful for genetic and forensic investigations.

research Frequency of an electrophoretic variant of hair alpha keratin in human populations.

14 citations , January 1977 in “PubMed”

The hair keratin variant is mostly found in Caucasians.

Electrophoretic Variability in Human Hair: Comparative Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of Body and Head Hair Proteins

research Electrophoretic variability in human hair: Comparative sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of body and head hair proteins

11 citations , January 1987 in “Electrophoresis”

Keratin proteins are consistent across different hair types from the same person.

research Chemical and photo-oxidative hair damage studied by dye diffusion and electrophoresis.

5 citations , November 2003 in “PubMed”

Chemical treatments and light exposure damage hair proteins.

research Electrophoretic analysis of human hair keratins

4 citations , December 1995 in “Anthropologischer Anzeiger”

Family members have similar hair protein patterns, which could be useful for genetic studies.

research Electrophoretic variation of hair proteins.

2 citations , November 1996 in “PubMed”

Most people have similar hair protein patterns, but a rare variant was found in two women.

research A genetic electrophoretic variant of high-sulfur hair proteins for forensic hair comparisons. II. Practical application of electrophoretic analysis of hair protein to forensic hair comparisons.

1 citations , February 1989 in “PubMed”

Electrophoresis can effectively analyze hair proteins for forensic use, even after cosmetic treatments and up to 2 years of weathering.

research Inheritance of Some Electrophoretic Phenotypes of Human Hair

January 1990 in “Advances in forensic haemogenetics”

Human hair protein patterns are inherited genetically.

research [Electrophoretic studies of acid mucopolysaccharides in skin, prekeratin of hair follicles and in keratoses of wool].

September 1978 in “PubMed”

Chondroitin sulphate is more abundant than hyaluronic acid in skin and hair samples, except in gamma-keratosis.

research A genetic electrophoretic variant of high-sulfur hair proteins for forensic hair comparisons. I. Characterization of variant high-sulfur proteins of human hair.

February 1989 in “PubMed”

A genetic hair protein variant is more common in Japanese people and is inherited.

Development and Validation of a Procedure for the Determination of Minoxidil in Hair-Regrowth Formulations Using Two Variants of Capillary Zone Electrophoresis

research Development and validation of a procedure for the determination of minoxidil in hair-regrowth formulations using two variants of capillary zone electrophoresis

14 citations , May 2005 in “Farmaco”

A method was created in 2005 to identify minoxidil, a hair growth ingredient, in products using two types of capillary zone electrophoresis, and it found that most products had about 2% minoxidil.

research Development of high-performance two-dimensional gel electrophoresis for human hair shaft proteome

13 citations , March 2019 in “PLoS ONE”

A new method improves protein analysis in hair, aiding health and disease research.

research Two-dimensional electrophoretic analysis of human hair keratins, especially hair matrix proteins

10 citations , January 1989 in “Archives of Dermatological Research”

The method effectively analyzes human hair proteins, especially nonfilamentous ones.

research Amino Acid Compositions of Human Hair Fibrous Protein Components Purified with Two‐dimensional Electrophoresis

4 citations , December 1989 in “The Journal of Dermatology”

Human hair proteins have similar cysteine and glycine levels to skin proteins.

research Improved two‐dimensional electrophoretic mapping of Japanese human hair proteins; application to curved and straight Japanese human hairs; and protein identification by MALDI MS and MS/MS quadrupole time‐of‐flight mass spectrometry

April 2020 in “International Journal of Cosmetic Science”

The study found that minor protein differences between curved and straight Japanese hair are unlikely to significantly affect hair structure.

A Study of the Soluble Proteins of Normal and Pathologic Horny Tissues by a Modified Disc Electrophoresis Technique

research A Study of the Soluble Proteins of Normal and Pathologic Horny Tissues by a Modified Disc Electrophoresis Technic

14 citations , November 1963 in “The journal of investigative dermatology/Journal of investigative dermatology”

Pathologic tissues have more soluble proteins than normal tissues.

research [Analysis of normal human hair keratins by two-dimensional polyacrylamide gel electrophoresis].

May 1988 in “PubMed”

research Analysis of the abnormal human hair keratins by two-dimensional polyacrylamide gel electrophoresis

March 1990 in “Journal of Dermatological Science”

research Krtap16, Characterization of a New Hair Keratin-associated Protein (KAP) Gene Complex on Mouse Chromosome 16 and Evidence for Regulation by Hoxc13

47 citations , September 2004 in “Journal of Biological Chemistry”

Hoxc13 regulates specific hair protein genes on mouse chromosome 16.

research Enzyme Typing of Human Hair Roots

27 citations , April 1978 in “Journal of Forensic Sciences”

Enzyme typing can reliably characterize human hair.

research Characterisation of low abundance wool proteins through novel differential extraction techniques

26 citations , June 2010 in “Electrophoresis”

New techniques helped identify rare wool proteins by reducing dominant ones.

research A Fatty Acid / Protein Complex in Human Hair

19 citations , March 1961 in “Nature”

A fatty acid/protein complex in human hair helps protect it from damage.

research In Vitro Methodologies to Evaluate the Effects of Hair Care Products on Hair Fiber

13 citations , January 2017 in “Cosmetics”

Different tests are used to see how hair care products affect hair, and choosing the right test is important for accurate results.

research Effect of procyandin oligomers on oxidative hair damage

10 citations , January 2011 in “Skin research and technology”

Procyanidin oligomers can protect hair from oxidative damage and could be good for hair care products.

research Simultaneous Typing of Erythrocyte Acid Phosphatase, Adenylate Kinase and Adenosine Deaminase in Human Hair Root Sheaths

1 citations , September 1986 in “Journal of the Forensic Science Society”

Hair root sheaths can be used to accurately analyze genetic markers.