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GlossaryFluorescence Microscopy

uses light to make specific cell parts glow for study

Fluorescence Microscopy is a technique that uses high-intensity light to excite fluorescent molecules in a sample, causing them to emit light at a different wavelength. This allows scientists to visualize and study specific components of cells and tissues with high specificity and contrast, making it particularly useful in biological research for observing structures like proteins, nucleic acids, and other cellular components.

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Multi-View Light Sheet Fluorescence Microscopy for Imaging Cellular Self-Assembly in Spheroids of Human Hair Follicle Dermal Papilla Cells and Keratinocytes

research 812 Multi-view light sheet florescence microscopy (LSFM) for imaging cellular self-assembly in spheroids of human hair follicle dermal papilla cells and keratinocytes

June 2020 in “Journal of Investigative Dermatology”

The technique effectively shows how human skin and hair cells form into ball-like structures.

research Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs ofArabidopsis thaliana

56 citations , March 2017 in “Plant and Cell Physiology”

Light sheet fluorescence microscopy effectively measures calcium changes in Arabidopsis root hairs.

research LB1605 3D imaging of cleared ex vivo normal human skin, skin appendages and psoriasiform skin lesion using light-sheet microscopy

August 2018 in “Journal of Investigative Dermatology”

The conclusion is that using light-sheet fluorescence microscopy with a special solution can effectively create detailed 3D images of human skin for dermatological research.

research Novel insights into hair structure and the effects of chemical stressors on hair and skin using label-free advanced light microscopy

January 2017 in “Durham e-Theses (Durham University)”

Chemical stressors damage hair structure but the skin's outer layer protects living cells.

research Photodegradation of human hair: a microscopy study

7 citations , January 2001 in “Comprehensive series in photosciences”

UV radiation significantly damages hair, especially with humidity, making it more vulnerable to further damage.

research FLIM Reveals Red Light-Induced Changes in Murine Hair Follicles

April 2026 in “Biosensors”

Red light increases hair follicles and ATP in mouse skin.

Unraveling Childhood Tinea Capitis: A Multi-Dimensional Investigation Using Dermoscopy, Scanning Electron Microscopy, and Mass Spectrometry

research Unravelling Childhood Tinea Capitis: A Multi-Dimensional Investigation Using Dermoscopy, Scanning Electron Microscopy and Mass Spectrometry

February 2024 in “Infection and drug resistance”

Tinea capitis in a child was caused by a fungus from cats, highlighting the need for accurate diagnosis and treatment.

research Cortical cell types and intermediate filament arrangements correlate with fiber curvature in Japanese human hair

62 citations , December 2008 in “Journal of structural biology”

Hair curvature in Japanese people is linked to specific cell types and filament arrangements in the hair cortex.

research Chemical and photo-oxidative hair damage studied by dye diffusion and electrophoresis.

5 citations , November 2003 in “PubMed”

Chemical treatments and light exposure damage hair proteins.

research Research Techniques Made Simple: Optical Clearing and Three-Dimensional Volumetric Imaging of Skin Biopsies

2 citations , June 2020 in “Journal of Investigative Dermatology”

3D imaging of skin biopsies offers better accuracy but is time-consuming and can't clear melanin.

research Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

97 citations , December 2021 in “Cells”

Designing effective fluorescence microscopy experiments requires careful consideration of hardware, biological models, and imaging agents.

research Elastic staining versus fluorescent and polarized microscopy in the diagnosis of alopecia

18 citations , May 2013 in “Journal of The American Academy of Dermatology”

EVG staining is the most reliable method for diagnosing alopecia.

research An Investigation of Hair and its Keratin Associated Proteins using Advanced Light Microscopy

January 2019 in “Durham e-Theses (Durham University)”

Advanced microscopy shows hair damage and keratin proteins' roles, aiding future cosmetic treatments.

research Live imaging of skin immunity using two-photon microscopy: a short review

January 2026 in “Inflammation and Regeneration”

Two-photon microscopy improves skin imaging but faces safety and cost challenges for clinical use.

research Two‐photon microscopy for intracutaneous imaging of stem cell activity in mice

16 citations , September 2016 in “Experimental Dermatology”

Two-photon microscopy effectively tracks live stem cell activity in mouse skin with minimal harm and clear images.

research Polarized microscopy in genetic hair disorders: case series

January 2025

Polarized microscopy helps identify hair irregularities in genetic disorders.

research Microscopic high-resolution digital volumetric imaging of human hair fibers

March 2005 in “International Journal of Cosmetic Science”

DVI provides detailed 3D imaging of hair and shows how various products protect and enhance hair.

research Use of fluorescent light energy for the management of bacterial skin infection associated with canine calcinosis cutis lesions

5 citations , October 2020 in “Veterinary record case reports”

Fluorescent light energy may help treat skin infections in dogs with calcinosis cutis.

research The Quantification of Terminal Hair by Digital Microscopy: Advancements Towards a More Objective Diagnosis of Hirsutism

November 2025 in “Clinical Cosmetic and Investigational Dermatology”

Digital microscopy can help diagnose hirsutism by measuring hair growth.

research Microscopy of the hair and trichogram

March 2014 in “Turkderm”

Hair microscopy is a useful and affordable way to diagnose hair disorders.

research Three-dimensional reconstructions of mechanosensory end organs suggest a unifying mechanism underlying dynamic, light touch

5 citations , March 2023 in “bioRxiv (Cold Spring Harbor Laboratory)”

Dynamic, light touch is sensed through a common mechanism involving Piezo2 channels in sensory axons.

research A Correlative Fluorescent and Electron Microscopic Technique for Ultralocalization of Trichocyte Keratins

1 citations , January 2021 in “Springer Proceedings in Materials”

Researchers developed a new method to clearly see and label hair proteins with minimal errors using advanced freezing and microscopy techniques.

research Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing

13 citations , September 2021 in “Communications Biology”

Co5M offers a new way to observe and understand wound healing without labels.

research Effect of disulfide bonds in human hair fibers on the melting behavior of their crystalline structure

March 2010 in “International Journal of Cosmetic Science”

Disulfide bonds affect the melting behavior of hair's crystalline structure, but hair retains some stability even after these bonds are broken.

research Qualitative investigation of fresh human scalp hair with full-field optical coherence tomography

10 citations , January 2012 in “Journal of biomedical optics”

Melanin density affects hair color, and this method can help in cosmetic assessments and diagnosing hair diseases.

research Spectroscopy as a tool to evaluate hair damage and protection

2 citations , November 2018 in “International Journal of Cosmetic Science”

Chamomile extract can protect hair from damage caused by light.

research In vivo multiphoton microscopy imaging of vitiligo (Conference Presentation)

March 2019

Multiphoton microscopy helps understand and improve vitiligo treatments by visualizing skin cell changes.

research 876 Imaging nanoscale changes in desmosome protein organization

April 2018 in “Journal of Investigative Dermatology”

Desmoglein 3 organization in cell connections changes without calcium, affecting cell adhesion.

research Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

4 citations , March 2013 in “InTech eBooks”

Confocal Laser Scanning Microscopy (CLSM) is a useful tool for studying how drugs interact with skin and diagnosing skin disorders, despite some limitations.

research Hair microscopy: an easy adjunct to diagnosis of systemic diseases in children

3 citations , November 2021 in “Applied Microscopy”

Hair microscopy is a simple and cost-effective method to help diagnose systemic diseases in children.