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This study introduces a natural hair-coloring system using size-controlled ink particles (SIPs) from cuttlefish ink and chitosan, addressing concerns about conventional dyes. The SIPs, initially negatively charged, were modified through polyelectrolyte complexation with chitosan to reverse their charge under acidic conditions, allowing effective electrostatic deposition onto hair fibers. The optimal SIP:chitosan weight ratio of 10:1 at pH ~4.7 produced the darkest and most uniform black coloration without using metallic mordants, surpassing the performance of plant-based natural colorants. This marine-biomass-derived method offers a promising alternative for safe and effective hair dyeing.

The document reviews the development and evaluation of natural hair dye formulations, highlighting the growing interest in plant-derived pigments like henna, indigo, and beetroot as safer alternatives to synthetic dyes. These natural dyes primarily coat the hair surface and are associated with fewer health risks, showing low irritation potential in studies. However, they face challenges such as limited shade range, application consistency, and fading with washing. Some commercial products may be adulterated with synthetic additives, affecting safety perceptions. The review emphasizes the need for further research on standardization and improving the performance and acceptance of natural hair dyes.

The document reviews the development and evaluation of natural hair dye formulations, highlighting the growing interest in plant-derived pigments like henna, indigo, and beetroot as safer alternatives to synthetic dyes. These natural dyes primarily coat the hair surface and are associated with fewer health risks, showing near-neutral pH, low irritation potential, and good stability. However, they face challenges such as limited shade range, application consistency, and fading with washing. Some commercial products may be adulterated with synthetic additives, affecting safety perceptions. The review emphasizes the need for further research on standardization and improved delivery systems for better consumer acceptance.