Search

for
Search:

GlossaryReverse Transcription Polymerase Chain Reaction

method to convert RNA into DNA and amplify it

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a laboratory technique used to convert RNA into DNA and then amplify specific DNA sequences. This method is crucial for studying gene expression, detecting RNA viruses, and diagnosing diseases, as it allows scientists to measure the amount of RNA in a sample by first transcribing it into complementary DNA (cDNA) and then amplifying it for analysis.

Related Terms

Sort by

Research

30 / 1000+ results

research Molecular mapping of lymph node metastases by real-time reverse transcription polymerase chain reaction in two melanoma patients

March 2011 in “Journal of Dermatology”

The conclusion is that a certain test might help find cancer spread in lymph nodes for melanoma patients, but more research is needed due to false positives.

Analysis of Gene Expression in Isolated Single Hair Follicles Using Semiquantitative Reverse-Transcriptase Polymerase Chain Reaction

research Analysis of Gene Expression in Isolated Single Hair Follicles: An Approach Using Semiquantitative Reverse-Transcriptase—Polymerase Chain Reaction

May 1995 in “Journal of Investigative Dermatology”

Researchers developed a new way to measure gene activity in single hair follicles and found that a specific gene's activity changes with different amounts and times of treatment.

research Clinical picture, diagnosis, treatment and outcome of severe acute respiratory syndrome (SARS) in children

42 citations , November 2004 in “Paediatric Respiratory Reviews”

Children generally have milder SARS symptoms than adults, with good outcomes and no deaths reported, but long-term effects are unclear.

research Identification and characterization of an antisense RNA transcript (gfg) from the human basic fibroblast growth factor gene.

24 citations , July 1994 in “Molecular Endocrinology”

Researchers found an RNA transcript that might help control a growth factor linked to tumor development.

research Analysis of Cytokines and ATP in Plucked Hair Follicles

8 citations , January 2021 in “Bulletin of Experimental Biology and Medicine”

ATP and IL-10 levels in hair follicles relate to follicle length and hair growth, with differences in alopecia patients.

research Expression of the RORα gene in Inner Mongolian cashmere goat hair follicles

7 citations , January 2015 in “Genetics and molecular research”

The RORα gene is active in different parts of cashmere goat hair follicles and may be influenced by melatonin, especially in December when hair growth changes.

research Extraction and Detection of mRNA from Horsehair

5 citations , January 2006 in “Journal of veterinary medical science”

RNA can be extracted from horsehair roots for analysis.

research Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium

4 citations , January 2016 in “Methods in molecular biology”

Hair follicle stem cells can become nerve cells using specific treatments.

research Analysis of LncRNA and mRNA Expression Profiles in Skin Tissues of Super Merino Sheep and Small-Tailed Han Sheep

November 2023

Researchers found genes in sheep that may affect hair growth and wool quality.

research Expression analysis of KAP9.2 and Hoxc13 genes during different cashmere growth stages by qRT-PCR method

9 citations , June 2014 in “Molecular biology reports”

KAP9.2 and Hoxc13 genes are important for cashmere growth and vary in activity during different stages.

research Identification and profiling of microRNA between back and belly Skin in Rex rabbits (Oryctolagus cuniculus)

1 citations , June 2018 in “World rabbit science”

Different miRNAs in Rex rabbit skin affect cell processes and hair growth.

research Skin-specific transgenic overexpression of ovine β-catenin in mice

2 citations , January 2023 in “Frontiers in Genetics”

Overexpressing ovine β-catenin in mice skin increases hair follicle density and growth.

research Interferon β Secreted from Human Hair Dermal Papilla Cells Inhibits the Growth of Outer Root Sheath Cells Cultured in Vitro

13 citations , January 2002 in “Biochemical and biophysical research communications”

Interferon β from hair cells stops the growth of other hair cells.

research URTICA DIOICA EXTRACT DOWNREGULATES THE GENE EXPRESSION OF 5Α-RII IN HACAT CELLS: POSSIBLE IMPLICATIONS AGAINST ANDROGENIC SKIN DISEASES.

1 citations , January 2023 in “PubMed”

Urtica dioica extract may help treat androgenic skin diseases by reducing a specific gene's activity.

research Mitogen‐regulated protein/proliferin mRNA induction following single applications of tumor promoters to murine skin

May 2005 in “Molecular Carcinogenesis”

mrp/plf-mRNA can indicate tumor-promoting effects in skin.

research Cyclin-dependent kinase inhibitors, p21waf1/cip1 and p27kip1, are expressed site- and hair cycle-dependently in rat hair follicles

9 citations , February 2001 in “Journal of Dermatological Science”

p21waf1/cip1 and p27kip1 help in hair follicle differentiation in rats.

research Analysis of gene expression patterns and levels in maize hybrids and their parents

5 citations , January 2015 in “Genetics and Molecular Research”

Maize hybrids show better early growth due to complex gene interactions from their parent strains.

research Association Between Human Hair Loss and the Expression Levels of Nucleolin, Nucleophosmin, andUBTFGenes

5 citations , February 2016 in “Genetic Testing and Molecular Biomarkers”

Hair loss is significantly linked to lower levels of certain genes in hair follicles.

EDA and EDAR Expression at Different Stages of Hair Follicle Development in Cashmere Goats and Effects on Expression of Related Genes

research EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes

12 citations , December 2020 in “Archives animal breeding/Archiv für Tierzucht”

EDA and EDAR are important for hair follicle development in cashmere goats and affect other related genes.

research Polymorphism in the A2M gene associated with high-quality milk in Murrah buffaloes (Bubalus bubalis)

5 citations , January 2016 in “Genetics and molecular research”

Certain genetic variations in the A2M gene are linked to better milk quality in Murrah buffaloes.

research Using WGCNA (weighted gene co-expression network analysis) to identify the hub genes of skin hair follicle development in fetus stage of Inner Mongolia cashmere goat

16 citations , December 2020 in “PloS one”

Researchers found WNT10A to be a key gene in developing goat hair follicles.

research Characterization and expression analysis of KAP7.1, KAP8.2 gene in Liaoning new-breeding cashmere goat hair follicle

48 citations , February 2010 in “Molecular biology reports”

KAP7.1 and KAP8.2 genes are crucial for cashmere quality in goats.

Trastuzumab Induced Chemobrain, Atorvastatin Rescued Chemobrain with Enhanced Anticancer Effect and without Hair Loss Side Effect

research Trastuzumab Induced Chemobrain, Atorvastatin Rescued Chemobrain with Enhanced Anticancer Effect and without Hair Loss-Side Effect

15 citations , February 2019 in “Journal of clinical medicine”

Atorvastatin reversed memory problems caused by cancer drug trastuzumab and improved its cancer-fighting abilities without causing hair loss.

Phloroglucinol Enhances Anagen Signaling and Alleviates H2O2-Induced Oxidative Stress in Human Dermal Papilla Cells

research Phloroglucinol Enhances Anagen Signaling and Alleviates H₂O₂-Induced Oxidative Stress in Human Dermal Papilla Cells

March 2024 in “Journal of Microbiology and Biotechnology”

Phloroglucinol may help improve hair loss by promoting hair growth and reducing oxidative stress.

Sebaceous Immunobiology: Skin Homeostasis, Innate Immunity Coordination, Inflammatory Response, and Disease Associations

research Sebaceous immunobiology - skin homeostasis, pathophysiology, coordination of innate immunity and inflammatory response and disease associations

21 citations , November 2022 in “Frontiers in immunology”

Sebaceous glands play a key role in skin health, immunity, and various skin diseases.

Red Ginseng Extract Promotes Hair Growth in Cultured Human Hair Follicles

research Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles

49 citations , November 2014 in “Journal of Medicinal Food”

Red Ginseng Extract may help human hair grow by activating growth pathways and blocking negative effects of certain hormones.

research Molecular Basis for Hair Loss in Mice Carrying a Novel Nonsense Mutation (Hr^rh-R) in the Hairless Gene (Hr)

10 citations , January 2010 in “Veterinary pathology”

A new mutation in the hairless gene causes hair loss and skin wrinkling in mice.

The Success of Thread-Embedding Therapy in Generating Hair Regrowth in Mice Points to Its Possible Effect in Humans

research The Success of Thread-embedding Therapy in Generating Hair Re-growth in Mice Points to Its Possibly Having a Similar Effect in Humans

20 citations , December 2015 in “Journal of Pharmacopuncture”

Thread-embedding therapy helped hair grow back in mice and might do the same in humans.

The Transcriptional Co-Activator cAMP Response Element-Binding Protein-Binding Protein Is Expressed in Prostate Cancer and Enhances Androgen- and Anti-Androgen-Induced Androgen Receptor Function

research The Transcriptional Co-Activator cAMP Response Element-Binding Protein-Binding Protein Is Expressed in Prostate Cancer and Enhances Androgen- and Anti-Androgen-Induced Androgen Receptor Function

72 citations , January 2003 in “American Journal of Pathology”

A protein called CBP is found in prostate cancer and can increase the effectiveness of certain prostate cancer treatments.

Identification of Transcriptional Targets of Wnt/β-Catenin Signaling in Dermal Papilla Cells of Human Scalp Hair Follicles: EP2 Is a Novel Transcriptional Target of Wnt3a

research Identification of transcriptional targets of Wnt/β-catenin signaling in dermal papilla cells of human scalp hair follicles: EP2 is a novel transcriptional target of Wnt3a

29 citations , March 2010 in “Journal of Dermatological Science”

Wnt3a activates certain genes in hair follicle cells, including a newly discovered one, EP2, which may affect hair growth.