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GlossaryCentrifugation

separates mixture components by spinning to sort densities

Centrifugation is a laboratory technique used to separate components of a mixture based on their density by spinning them at high speeds. When a sample is placed in a centrifuge, the centrifugal force pushes denser particles to the bottom of the container, while less dense particles remain closer to the top, allowing for efficient separation and analysis of different substances.

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research The Impact of Centrifugation Devices and Collection Tubes on Fibrin Characteristics and Growth Factor Release Under High- and Low-Speed Protocols

3 citations , October 2025 in “Dentistry Journal”

Low-speed protocols with certified centrifuges and FDA-approved glass tubes are best for effective PRF outcomes.

A Cross-Sectional Study Optimizing Platelet-Rich Plasma Preparation Using Various Centrifugation Speeds and Its Effects on the Platelet Yield

research A cross sectional study optimizing platelet rich plasma preparation using various centrifugation speeds and its effects on the platelet yield

September 2024 in “IP Indian Journal of Clinical and Experimental Dermatology”

The best method for high platelet yield is centrifuging at 1300 rpm then 1600 rpm.

research Effect of 10% calcium gluconate on the activation of platelet-rich plasma obtained at different centrifugation conditions on the expression of platelet derived growth factor-BB (PDGF-BB)

January 2024 in “Universidad Peruana Cayetano Heredia Institutional Repository”

Simple centrifugation with calcium gluconate boosts PDGF-BB release in PRP.

research Impact of the Different Preparation Methods to Obtain Human Adipose-Derived Stromal Vascular Fraction Cells (AD-SVFs) and Human Adipose-Derived Mesenchymal Stem Cells (AD-MSCs): Enzymatic Digestion Versus Mechanical Centrifugation

124 citations , November 2019 in “International Journal of Molecular Sciences”

Standardized kits improve the quality and consistency of isolating stem cells from fat tissue.

research The effect of different speeds of centrifugation on platelet-rich plasma preparation

2 citations , September 2015 in “Journal of the Egyptian Women's Dermatologic Society (Print)”

Different centrifugation speeds change the quality of platelet-rich plasma.

research Enhancement of Progenitor Cells by Two-Step Centrifugation of Emulsified Lipoaspirates

2 citations , March 2019 in “Plastic and Reconstructive Surgery”

The letter suggests that a modified fat processing technique may increase regenerative cells but calls for more trials to confirm its effectiveness for skin and hair treatments.

Comparison of Single-Spin to Double-Spin Platelet-Rich Plasma Centrifugation Methods in the Treatment of Androgenic Alopecia: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

research Comparison of single-spin to double-spin platelet-rich plasma centrifugation methods in the treatment of androgenic alopecia: a systematic review and meta-analysis of randomized controlled trials

July 2025 in “Frontiers in Medicine”

Single-spin and double-spin PRP methods are equally effective for treating hair loss.

research Supplementary file 1_Comparison of single-spin to double-spin platelet-rich plasma centrifugation methods in the treatment of androgenic alopecia: a systematic review and meta-analysis of randomized controlled trials.pdf

July 2025 in “OPAL (Open@LaTrobe) (La Trobe University)”

Single-spin PRP may be more effective for hair loss, but more research is needed.

research Supplementary file 2_Comparison of single-spin to double-spin platelet-rich plasma centrifugation methods in the treatment of androgenic alopecia: a systematic review and meta-analysis of randomized controlled trials.docx

July 2025 in “OPAL (Open@LaTrobe) (La Trobe University)”

Single-spin PRP may be more effective for treating hair loss.

Comparing Single-Spin Versus Double-Spin Platelet-Rich Plasma Centrifugation Methods on Thrombocyte Count and Clinical Improvement of Androgenetic Alopecia: A Preliminary, Randomized, Double-Blind Clinical Trial

research Comparing Single-spin Versus Double-spin Platelet-rich Plasma (PRP) Centrifugation Methods on Thrombocyte Count and Clinical Improvement of Androgenetic Alopecia: A Preliminary, Randomized, Double-blind Clinical Trial.

December 2023 in “PubMed”

Both single-spin and double-spin PRP methods effectively treat hair loss, with single-spin slightly increasing platelet count more.

research Mechanical Fractionation of Adipose Tissue—A Scoping Review of Procedures to Obtain Stromal Vascular Fraction

19 citations , October 2023 in “Bioengineering”

tSVF is effective for treating inflammation-related conditions, with centrifugation being the best method for isolation.

research Platelet-Therapeutics to Improve Tissue Regeneration and Wound Healing—Physiological Background and Methods of Preparation

14 citations , July 2021 in “Biomedicines”

Platelet-rich treatments can help improve wound healing and tissue repair.

research Technical Procedures for Preparation and Administration of Platelet-Rich Plasma and Related Products: A Scoping Review

12 citations , December 2020 in “Frontiers in Cell and Developmental Biology”

The review found that the way Platelet-Rich Plasma is made varies a lot, which can change the results of medical treatments.

research Variables affecting the potential efficacy of platelet-rich plasma in dermatology

9 citations , August 2020 in “Journal of The American Academy of Dermatology”

Many factors, like patient health and how PRP is made, can affect how well PRP therapy works in skin treatments.

research Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells

8 citations , January 2017 in “Methods in molecular biology”

Stem cells rearrangement regenerates functional hair follicles, potentially treating hair loss.

Fat Transfer for the Management of Soft Tissue Trauma: The Do's and Don'ts

research Fat Transfer for the Management of Soft Tissue Trauma: The Do's and the Don'ts

8 citations , November 2010 in “Facial Plastic Surgery”

Fat transfer can be effective for soft tissue trauma if the patient is chosen carefully and the procedure is done correctly.

research Minimum requirements for reporting clinical trials evaluating the therapeutic potential of platelet-rich plasma

7 citations , August 2020 in “Journal of The American Academy of Dermatology”

The document sets guidelines to standardize reporting of PRP clinical trials for better reproducibility and comparability.

research Platelet-Rich Plasma in Cosmetic Surgery

5 citations , January 2013 in “Otorhinolaryngology clinics : an international journal”

Platelet-rich plasma may improve healing and hair growth in cosmetic surgery but results vary.

research Rise of stem cell therapies in aesthetics

4 citations , August 2021 in “Clinics in Dermatology”

Stem cells show promise for hair loss and skin treatments in aesthetics but need more research on safety and standard methods.

Autologous Platelet-Rich Plasma: A Review of Scientific Articles on the Study of Efficiency in Treatment of Androgenetic Alopecia in Men and Women

research AUTOLOGOUS PLATELET‐RICH PLASMA: A REVIEW OF SCIENTIFIC ARTICLES ON THE STUDY OF EFFICIENCY IN TREATMENT OF ANDROGENETIC ALOPECIA IN MEN AND WOMEN

1 citations , January 2018 in “Journal of V. N. Karazin Kharkiv National University: Series Medicine”

Platelet-rich plasma can positively affect common hair loss, but more research is needed for standard use.

Anticoagulant-Free Preparation of Autologous Platelet-Rich Plasma (PRP) / Fluid Platelet-Rich Fibrin (f-PRF): A Pre-Clinical Comparative Performance Study

research Anticoagulant-free preparation of autologous platelet-rich plasma (PRP) / fluid platelet-rich fibrin (f-PRF): a pre-clinical comparative performance study

March 2026 in “Frontiers in Pharmacology”

Exprecell™ is as effective as traditional methods but produces more f-PRF and is simpler to use.

research Platelet‐rich fibrin: A review of its role as a new treatment in androgenetic alopecia

April 2024 in “Journal of cosmetic dermatology”

Platelet-rich fibrin might be a promising treatment for hair loss, but more research is needed to confirm its effectiveness.

Efficacy of Platelet-Rich Plasma in Androgenetic Alopecia and Correlation Between Platelet Count in PRP With Clinical and Trichoscopic Improvement

research EFFICACY OF PLATELET-RICH PLASMA IN ANDROGENETIC ALOPECIA AND CORRELATION BETWEEN PLATELET COUNT IN PRP WITH CLINICAL AND TRICHOSCOPIC IMPROVEMENT.

March 2024 in “INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH”

PRP can effectively treat hair loss, especially with optimal platelet count.

research Use of Plasma Gel as Autologous Collagen Biostimulator with Simplified Technique: Case Report

December 2023

Plasma gel and PRP treatments improve skin and hair with minimal side effects.

research Does Platelet‐Rich Plasma Have a Therapeutic Role in Hair Restoration?

January 2023 in “Laryngoscope”

Platelet-rich plasma (PRP) can help restore hair, but the best method for preparing and using it is still unclear.

Platelet-Rich Plasma And Platelet Fibrin: Standardized Preparation Protocols, Mechanisms Of Activation, Storage Stability, And Regulatory Frameworks

research PLATELET-RICH PLASMA AND PLATELET FIBRIN: STANDARDIZED PREPARATION PROTOCOLS, MECHANISMS OF ACTIVATION, STORAGE STABILITY, AND REGULATORY FRAMEWORKS

December 2025 in “Eastern Ukrainian Medical Journal”

Standardized protocols are crucial for effective use of platelet-rich plasma and fibrin in tissue regeneration.

research Efficacy of Autologous Activated Platelet-Rich Plasma (AA-PRP) in Androgenetic Alopecia: A Systematic Review and Meta-Analysis

January 2025 in “Open Science Framework”

AA-PRP temporarily increases hair density in androgenetic alopecia but loses effectiveness over time.

research PLATELET RICH FIBRIN- A Natural Regenerative Biomaterial

October 2023 in “Journal of Advanced Sciences”

Platelet Rich Fibrin (PRF) is a safe, effective tool for tissue regeneration and healing in various medical fields.

Formulation and Effectiveness Test of O/W Nanoemulsion of Candlenut Oil (Aleurites Moluccana (L)) on Hair Growth Rate in Male White Rats

research Formulasi dan Uji Efektivitas Sediaan Nanoemulsi O/W Minyak Kemiri (Aleurites moluccana (L)) Terhadap Laju Pertumbuhan Rambut pada Tikus Putih Jantan

January 2020

Candlenut oil nanoemulsion increases hair growth in male rats and remains stable for 14 days at room temperature.

research Different platelet‐rich plasma preparation protocols in Female pattern hair loss: Does it affect the outcome? A pilot study

January 2022 in “Journal of Cosmetic Dermatology”

The best way to prepare Platelet-Rich Plasma for hair loss treatment in women is using a digital centrifuge, large sodium citrate tubes, and low speed spinning. This method is safe and effectively increases hair thickness and growth.